OAT ELISA Kit

Catalog No. ABIN2044137

This Human OAT ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human OAT.

Quick Overview for OAT ELISA Kit (ABIN2044137)

Target: OAT (Ornithine Aminotransferase (OAT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details OAT ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.059 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ornithine Aminotransferase (OAT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Aminotransferase (OAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Aminotransferase (OAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Aminotransferase (OAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ornithine Aminotransferase (OAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for OAT

Target: OAT (Ornithine Aminotransferase (OAT))