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Osteocalcin ELISA Kit

Chicken Osteocalcin ELISA Kit Colorimetric assay for quantification of Chicken Osteocalcin.
Catalog No. ABIN2044141

Quick Overview for Osteocalcin ELISA Kit (ABIN2044141)

Target

See all Osteocalcin (BGLAP) ELISA Kits
Osteocalcin (BGLAP)

Reactivity

  • 11
  • 10
  • 6
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  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Chicken

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

1.56-100 pg/mL

Application

ELISA
  • Minimum Detection Limit

    1.56 pg/mL

    Sensitivity

    < 0.67 pg/mL
  • Plate

    Pre-coated

    Protocol

    Osteocalcin (OC) BioAssay-ELISA Kit (Chicken) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin (OC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteocalcin (OC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteocalcin (OC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Osteocalcin (OC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all Osteocalcin (BGLAP) ELISA Kits

    Osteocalcin (BGLAP)

    Alternative Name

    Osteocalcin (OC)
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