SP7 ELISA Kit
Quick Overview for SP7 ELISA Kit (ABIN2044149)
Target
See all SP7 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.064 ng/mL
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Sp7 Transcription Factor (SP7) ELISA Kit
SP7 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Sp7 Transcription Factor (SP7) ELISA KitSP7 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Osterix (OSX) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osterix (OSX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osterix (OSX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osterix (OSX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Osterix (OSX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- SP7 (Sp7 Transcription Factor (SP7))
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Alternative Name
- Osterix (OSX)
Target See all SP7 ELISA Kits
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