Paralemmin ELISA Kit

Catalog No. ABIN2044167

This Human Paralemmin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Paralemmin.

Quick Overview for Paralemmin ELISA Kit (ABIN2044167)

Target: Paralemmin (PALM)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details Paralemmin ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Paralemmin (PALM) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paralemmin (PALM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paralemmin (PALM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paralemmin (PALM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Paralemmin (PALM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Paralemmin

Target: Paralemmin (PALM)

Pathways: cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling