PARK7/DJ1 ELISA Kit

Catalog No. ABIN2044171

Human PARK7/DJ1 ELISA Kit Colorimetric assay for quantification of Human PARK7/DJ1.

Quick Overview for PARK7/DJ1 ELISA Kit (ABIN2044171)

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details PARK7/DJ1 ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.64 ng/mL

Application Details

Plate: Pre-coated

Protocol: Parkinson Disease Protein 7 (PARK7) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Parkinson Disease Protein 7 (PARK7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Parkinson Disease Protein 7 (PARK7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Parkinson Disease Protein 7 (PARK7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Parkinson Disease Protein 7 (PARK7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PARK7/DJ1

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Alternative Name: Parkinson Disease Protein 7 (PARK7)

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport