PNPLA3 ELISA Kit

Catalog No. ABIN2044177

Rat PNPLA3 ELISA Kit Colorimetric assay for quantification of Rat PNPLA3.

Quick Overview for PNPLA3 ELISA Kit (ABIN2044177)

Target: PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details PNPLA3 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PNPLA3

Target: PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))

Alternative Name: Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3)