KIAA0101 ELISA Kit

Catalog No. ABIN2044179

This Human KIAA0101 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human KIAA0101.

Quick Overview for KIAA0101 ELISA Kit (ABIN2044179)

Target: KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details KIAA0101 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.27 ng/mL

Application Details

Plate: Pre-coated

Protocol: PCNA Associated Factor 15kDa (PAF) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCNA Associated Factor 15kDa (PAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PCNA Associated Factor 15kDa (PAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PCNA Associated Factor 15kDa (PAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of PCNA Associated Factor 15kDa (PAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for KIAA0101

Target: KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))

Alternative Name: PCNA Associated Factor 15kDa (PAF)

Pathways: Chromatin Binding