PGC ELISA Kit
Quick Overview for PGC ELISA Kit (ABIN2044183)
Target
See all PGC ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.063 ng/mL
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Pepsinogen C (PGC) ELISA Kit
PGC Reactivity: Human Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
Pepsinogen C (PGC) ELISA KitPGC Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Pepsinogen C (PGC) ELISA KitPGC Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Pepsinogen C (PGC) ELISA KitPGC Reactivity: Human Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
Pepsinogen C (PGC) ELISA KitPGC Reactivity: Human Colorimetric Sandwich ELISA 0.5-100 ng/mL Serum
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Plate
- Pre-coated
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Protocol
- Pepsinogen C (PGC) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsinogen C (PGC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pepsinogen C (PGC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pepsinogen C (PGC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pepsinogen C (PGC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- PGC (Pepsinogen C (PGC))
Target See all PGC ELISA Kits
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