PPARA ELISA Kit
Quick Overview for PPARA ELISA Kit (ABIN2044205)
Target
See all PPARA ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.31 ng/mL
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Sensitivity
- < 0.113 ng/mL
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Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA Kit
PPARA Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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Plate
- Pre-coated
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Protocol
- Peroxisome Proliferator Activated Receptor Alpha (PPARa) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Alpha (PPARa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Peroxisome Proliferator Activated Receptor Alpha (PPARa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
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Alternative Name
- Peroxisome Proliferator Activated Receptor alpha (PPARa)
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Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C
Target See all PPARA ELISA Kits
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