PEBP1 ELISA Kit

Catalog No. ABIN2044209

This Mouse PEBP1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse PEBP1.

Quick Overview for PEBP1 ELISA Kit (ABIN2044209)

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details PEBP1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.055 ng/mL

Application Details

Plate: Pre-coated

Protocol: Phosphatidylethanolamine Binding Protein 1 (PEBP1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylethanolamine Binding Protein 1 (PEBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphatidylethanolamine Binding Protein 1 (PEBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidylethanolamine Binding Protein 1 (PEBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phosphatidylethanolamine Binding Protein 1 (PEBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Pathways: Feeding Behaviour