PLA2G3 ELISA Kit

Catalog No. ABIN2044217

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse PLA2G3.

Quick Overview for PLA2G3 ELISA Kit (ABIN2044217)

Target: PLA2G3 (Phospholipase A2, Group III (PLA2G3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details PLA2G3 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Brand: BioAssay™

Sensitivity: < 0.27 ng/mL

Application Details

Plate: Pre-coated

Protocol: Phospholipase A2, Group III (PLA2G3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group III (PLA2G3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group III (PLA2G3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group III (PLA2G3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phospholipase A2, Group III (PLA2G3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PLA2G3

Target: PLA2G3 (Phospholipase A2, Group III (PLA2G3))