PIM3 ELISA Kit

Catalog No. ABIN2044229

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PIM3.

Quick Overview for PIM3 ELISA Kit (ABIN2044229)

Target: PIM3 (Pim-3 Oncogene (PIM3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.62-40 ng/mL

Application: ELISA

Product Details PIM3 ELISA Kit

Minimum Detection Limit: 0.62 ng/mL

Sensitivity: < 0.228 ng/mL

Application Details

Plate: Pre-coated

Protocol: Pim-3 Oncogene (PIM3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pim-3 Oncogene (PIM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pim-3 Oncogene (PIM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pim-3 Oncogene (PIM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pim-3 Oncogene (PIM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PIM3

Target: PIM3 (Pim-3 Oncogene (PIM3))

Pathways: Negative Regulation of Hormone Secretion