PDGFD ELISA Kit

Catalog No. ABIN2044241

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat PDGFD.

Quick Overview for PDGFD ELISA Kit (ABIN2044241)

Target: PDGFD (Platelet Derived Growth Factor D (PDGFD))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details PDGFD ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.125 ng/mL

Application Details

Plate: Pre-coated

Protocol: Platelet Derived Growth Factor D (PDGFD) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor D (PDGFD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor D (PDGFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor D (PDGFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Platelet Derived Growth Factor D (PDGFD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PDGFD

Target: PDGFD (Platelet Derived Growth Factor D (PDGFD))

Pathways: RTK Signaling, Platelet-derived growth Factor Receptor Signaling