PARN ELISA Kit

Catalog No. ABIN2044251

This Human PARN ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human PARN.

Quick Overview for PARN ELISA Kit (ABIN2044251)

Target: PARN (Poly A Specific Ribonuclease (PARN))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details PARN ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.27 ng/mL

Application Details

Plate: Pre-coated

Protocol: Poly A Specific Ribonuclease (PARN) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poly A Specific Ribonuclease (PARN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poly A Specific Ribonuclease (PARN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poly A Specific Ribonuclease (PARN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Poly A Specific Ribonuclease (PARN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PARN

Target: PARN (Poly A Specific Ribonuclease (PARN))