PNOC ELISA Kit
Quick Overview for PNOC ELISA Kit (ABIN2044295)
Target
See all PNOC ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.058 ng/mL
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Prepronociceptin (PNOC) ELISA Kit
PNOC Reactivity: Rat Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Prepronociceptin (PNOC) ELISA KitPNOC Reactivity: Human Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Prepronociceptin (PNOC) ELISA KitPNOC Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Pronociceptin (PNOC) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pronociceptin (PNOC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pronociceptin (PNOC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pronociceptin (PNOC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pronociceptin (PNOC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- PNOC (Prepronociceptin (PNOC))
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Alternative Name
- Pronociceptin (PNOC)
Target See all PNOC ELISA Kits
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