PSMA5 ELISA Kit

Catalog No. ABIN2044319

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PSMA5.

Quick Overview for PSMA5 ELISA Kit (ABIN2044319)

Target: PSMA5 (Proteasome Subunit alpha 5 (PSMA5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details PSMA5 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.114 ng/mL

Application Details

Plate: Pre-coated

Protocol: Proteasome Subunit Alpha Type 5 (PSMa5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Subunit Alpha Type 5 (PSMa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteasome Subunit Alpha Type 5 (PSMa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Subunit Alpha Type 5 (PSMa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Proteasome Subunit Alpha Type 5 (PSMa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PSMA5

Target: PSMA5 (Proteasome Subunit alpha 5 (PSMA5))

Alternative Name: Proteasome Subunit alpha Type 5 (PSMa5)

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA