PKN1 ELISA Kit

Catalog No. ABIN2044339

Human PKN1 ELISA Kit Colorimetric assay for quantification of Human PKN1.

Quick Overview for PKN1 ELISA Kit (ABIN2044339)

Target: PKN1 (Protein Kinase N1 (PKN1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details PKN1 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.117 ng/mL

Application Details

Plate: Pre-coated

Protocol: Protein Kinase N1 (PKN1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase N1 (PKN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase N1 (PKN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase N1 (PKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Kinase N1 (PKN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PKN1

Target: PKN1 (Protein Kinase N1 (PKN1))