Protein Kinase, X-Linked (PRKX) ELISA Kit

Details for Product No. ABIN2044345
Target
Reactivity
Rat
Alternatives
Kits with alternative reactivity to:
2
1
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
1.56-100 ng/mL
Minimum Detection Limit
1.56 ng/mL
Application
ELISA
Options
Brand BioAssay™
Detection Method Colorimetric
Sensitivity < 0.60 ng/mL
Target
Pathways Thyroid Hormone Synthesis
Plate Pre-coated
Protocol Protein Kinase, X-Linked (PRKX) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, X-Linked (PRKX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, X-Linked (PRKX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, X-Linked (PRKX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Kinase, X-Linked (PRKX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C