PRKX ELISA Kit
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- Target See all PRKX ELISA Kits
- PRKX (Protein Kinase, X-Linked (PRKX))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 1.56-100 ng/mL
- Minimum Detection Limit
- 1.56 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.60 ng/mL
- Top Product
- Discover our top product PRKX ELISA Kit
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- Plate
- Pre-coated
- Protocol
- Protein Kinase, X-Linked (PRKX) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, X-Linked (PRKX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, X-Linked (PRKX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, X-Linked (PRKX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Kinase, X-Linked (PRKX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all PRKX ELISA Kits
- PRKX (Protein Kinase, X-Linked (PRKX))
- Abstract
- PRKX Products
- Pathways
- Thyroid Hormone Synthesis
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