PC ELISA Kit

Catalog No. ABIN2044367

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PC.

Quick Overview for PC ELISA Kit (ABIN2044367)

Target: PC (Pyruvate Carboxylase (PC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details PC ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.63 ng/mL

Application Details

Plate: Pre-coated

Protocol: Pyruvate Carboxylase (PC) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Carboxylase (PC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Carboxylase (PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Carboxylase (PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pyruvate Carboxylase (PC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))