RAD51 Homolog ELISA Kit

Catalog No. ABIN2044379

This Colorimetric ELISA kit is designed for the quantitative measurement of Human RAD51 Homolog.

Quick Overview for RAD51 Homolog ELISA Kit (ABIN2044379)

Target: RAD51 Homolog (RAD51)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details RAD51 Homolog ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.081 ng/mL

Application Details

Plate: Pre-coated

Protocol: RAD51 Homolog (RAD51) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAD51 Homolog (RAD51). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAD51 Homolog (RAD51). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAD51 Homolog (RAD51), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of RAD51 Homolog (RAD51) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RAD51 Homolog

Target: RAD51 Homolog (RAD51)