RAC1 ELISA Kit

Catalog No. ABIN2044385

This Colorimetric ELISA kit is designed for the quantitative measurement of Human RAC1.

Quick Overview for RAC1 ELISA Kit (ABIN2044385)

Target: RAC1

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details RAC1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.060 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Related C3 Botulinum Toxin Substrate 1 (Rac1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RAC1

Target: RAC1

Alternative Name: Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling