RETNLB ELISA Kit
Quick Overview for RETNLB ELISA Kit (ABIN2044425)
Target
See all RETNLB ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 3.12 pg/mL
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Brand
- BioAssay™
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Sensitivity
- < 1.44 pg/mL
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Resistin Like beta (RETNLB) ELISA Kit
RETNLB Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Resistin Like beta (RETNLB) ELISA KitRETNLB Reactivity: Mouse Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum
Resistin Like beta (RETNLB) ELISA KitRETNLB Reactivity: Human Colorimetric Sandwich ELISA 47 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma, Serum
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Plate
- Pre-coated
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Protocol
- Resistin Like Beta (RETNLb) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin Like Beta (RETNLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin Like Beta (RETNLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin Like Beta (RETNLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Resistin Like Beta (RETNLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- RETNLB (Resistin Like beta (RETNLB))
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Pathways
- Hormone Activity
Target See all RETNLB ELISA Kits
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