RETNLB ELISA Kit

Catalog No. ABIN2044425

The Mouse RETNLB ELISA Kit (ABIN2044425) is a Colorimetric ELISA Kit designed to quantify Mouse RETNLB.

Quick Overview for RETNLB ELISA Kit (ABIN2044425)

Target: RETNLB (Resistin Like beta (RETNLB))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12-200 pg/mL

Application: ELISA

Product Details RETNLB ELISA Kit

Minimum Detection Limit: 3.12 pg/mL

Sensitivity: < 1.44 pg/mL

Application Details

Plate: Pre-coated

Protocol: Resistin Like Beta (RETNLb) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin Like Beta (RETNLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin Like Beta (RETNLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin Like Beta (RETNLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Resistin Like Beta (RETNLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RETNLB

Target: RETNLB (Resistin Like beta (RETNLB))

Pathways: Hormone Activity