Retinoid X Receptor gamma ELISA Kit

Catalog No. ABIN2044431

This Human Retinoid X Receptor gamma ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Retinoid X Receptor gamma.

Quick Overview for Retinoid X Receptor gamma ELISA Kit (ABIN2044431)

Target: Retinoid X Receptor gamma (RXRG) (Retinoid X Receptor, gamma (RXRG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details Retinoid X Receptor gamma ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Brand: BioAssay™

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Retinoid X Receptor Gamma (RXRg) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoid X Receptor Gamma (RXRg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoid X Receptor Gamma (RXRg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoid X Receptor Gamma (RXRg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Retinoid X Receptor Gamma (RXRg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Retinoid X Receptor gamma

Target: Retinoid X Receptor gamma (RXRG) (Retinoid X Receptor, gamma (RXRG))

Alternative Name: Retinoid X Receptor gamma (RXRg)

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway