RHD ELISA Kit

Catalog No. ABIN2044433

This Colorimetric ELISA kit is designed for the quantitative measurement of Human RHD.

Quick Overview for RHD ELISA Kit (ABIN2044433)

Target: RHD (Rh Blood Group, D Antigen (RHD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details RHD ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.058 ng/mL

Application Details

Plate: Pre-coated

Protocol: Rh Blood Group, D Antigen (RHD) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rh Blood Group, D Antigen (RHD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rh Blood Group, D Antigen (RHD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rh Blood Group, D Antigen (RHD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Rh Blood Group, D Antigen (RHD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RHD

Target: RHD (Rh Blood Group, D Antigen (RHD))