RNASE3 ELISA Kit

Catalog No. ABIN2044447

Human RNASE3 ELISA Kit Colorimetric assay for quantification of Human RNASE3.

Quick Overview for RNASE3 ELISA Kit (ABIN2044447)

Target: RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 39.06-2500 pg/mL

Application: ELISA

Product Details RNASE3 ELISA Kit

Minimum Detection Limit: 39.06 pg/mL

Sensitivity: < 17 pg/mL

Application Details

Plate: Pre-coated

Protocol: Ribonuclease A3 (RNASE3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A3 (RNASE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A3 (RNASE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A3 (RNASE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease A3 (RNASE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RNASE3

Target: RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))

Alternative Name: Ribonuclease A3 (RNASE3)