RNASE8 ELISA Kit

Catalog No. ABIN2044453

Human RNASE8 ELISA Kit Colorimetric assay for quantification of Human RNASE8.

Quick Overview for RNASE8 ELISA Kit (ABIN2044453)

Target: RNASE8 (Ribonuclease, RNase A Family, 8 (RNASE8))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details RNASE8 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.109 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ribonuclease A8 (RNASE8) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A8 (RNASE8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A8 (RNASE8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A8 (RNASE8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease A8 (RNASE8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RNASE8

Target: RNASE8 (Ribonuclease, RNase A Family, 8 (RNASE8))

Alternative Name: Ribonuclease A8 (RNASE8)