RNASET2 ELISA Kit

Catalog No. ABIN2044461

This Human RNASET2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human RNASET2.

Quick Overview for RNASET2 ELISA Kit (ABIN2044461)

Target: RNASET2 (Ribonuclease T2 (RNASET2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details RNASET2 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Brand: BioAssay™

Sensitivity: < 0.069 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ribonuclease T2 (RNASET2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease T2 (RNASET2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease T2 (RNASET2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease T2 (RNASET2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease T2 (RNASET2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RNASET2

Target: RNASET2 (Ribonuclease T2 (RNASET2))