SEMG2 ELISA Kit

Catalog No. ABIN2044525

Human SEMG2 ELISA Kit Colorimetric assay for quantification of Human SEMG2.

Quick Overview for SEMG2 ELISA Kit (ABIN2044525)

Target: SEMG2 (Semenogelin II (SEMG2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details SEMG2 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.114 ng/mL

Application Details

Plate: Pre-coated

Protocol: Semenogelin II (SEMG2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semenogelin II (SEMG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semenogelin II (SEMG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semenogelin II (SEMG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Semenogelin II (SEMG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SEMG2

Target: SEMG2 (Semenogelin II (SEMG2))