SPTLC3 ELISA Kit

Catalog No. ABIN2044529

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse SPTLC3.

Quick Overview for SPTLC3 ELISA Kit (ABIN2044529)

Target: SPTLC3 (Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details SPTLC3 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.061 ng/mL

Application Details

Plate: Pre-coated

Protocol: Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SPTLC3

Target: SPTLC3 (Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3))