SH3BP2 ELISA Kit

Catalog No. ABIN2044545

This Human SH3BP2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human SH3BP2.

Quick Overview for SH3BP2 ELISA Kit (ABIN2044545)

Target: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details SH3BP2 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Brand: BioAssay™

Sensitivity: < 0.126 ng/mL

Application Details

Plate: Pre-coated

Protocol: SH3 Domain Binding Protein 2 (SH3BP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SH3 Domain Binding Protein 2 (SH3BP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SH3 Domain Binding Protein 2 (SH3BP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SH3 Domain Binding Protein 2 (SH3BP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of SH3 Domain Binding Protein 2 (SH3BP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SH3BP2

Target: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Alternative Name: SH3 Domain Binding Protein 2 (SH3BP2)

Pathways: TCR Signaling