CD33 ELISA Kit

Catalog No. ABIN2044551

This Mouse CD33 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse CD33.

Quick Overview for CD33 ELISA Kit (ABIN2044551)

Target: CD33

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details CD33 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Sialic Acid Binding Ig Like Lectin 3 (SIGLEC3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialic Acid Binding Ig Like Lectin 3 (SIGLEC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sialic Acid Binding Ig Like Lectin 3 (SIGLEC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sialic Acid Binding Ig Like Lectin 3 (SIGLEC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Sialic Acid Binding Ig Like Lectin 3 (SIGLEC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CD33

Target: CD33

Alternative Name: Sialic Acid Binding Ig Like Lectin 3 (Siglec 3)