SIRPA ELISA Kit

Catalog No. ABIN2044553

This Colorimetric ELISA kit is designed for the quantitative measurement of Human SIRPA.

Quick Overview for SIRPA ELISA Kit (ABIN2044553)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details SIRPA ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.061 ng/mL

Application Details

Plate: Pre-coated

Protocol: Signal Regulatory Protein Alpha (SIRPa) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Regulatory Protein Alpha (SIRPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Regulatory Protein Alpha (SIRPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Regulatory Protein Alpha (SIRPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Signal Regulatory Protein Alpha (SIRPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: Signal Regulatory Protein alpha (SIRPa)