S1PR3 ELISA Kit

Catalog No. ABIN2044595

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse S1PR3.

Quick Overview for S1PR3 ELISA Kit (ABIN2044595)

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Application: ELISA

Product Details S1PR3 ELISA Kit

Minimum Detection Limit: 78.12 pg/mL

Sensitivity: < 27 pg/mL

Application Details

Plate: Pre-coated

Protocol: Sphingosine 1 Phosphate Receptor 3 (S1PR3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 3 (S1PR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 3 (S1PR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 3 (S1PR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Sphingosine 1 Phosphate Receptor 3 (S1PR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for S1PR3

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Alternative Name: Sphingosine 1 Phosphate Receptor 3 (S1PR3)