SPON2 ELISA Kit
Quick Overview for SPON2 ELISA Kit (ABIN2044601)
Target
See all SPON2 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
-
-
Minimum Detection Limit
- 0.15 ng/mL
-
Sensitivity
- < 0.069 ng/mL
-
-
Spondin 2 (SPON2) ELISA Kit
SPON2 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Spondin 2 (SPON2) ELISA KitSPON2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Spondin 2 (SPON2) ELISA KitSPON2 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Spondin 2 (SPON2) ELISA KitSPON2 Reactivity: Human Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Serum
-
-
Plate
- Pre-coated
-
Protocol
- Spondin 2 (SPON2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spondin 2 (SPON2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spondin 2 (SPON2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spondin 2 (SPON2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Spondin 2 (SPON2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C/-20 °C
-
-
- SPON2 (Spondin 2 (SPON2))
Target See all SPON2 ELISA Kits
-
