SNAP25 ELISA Kit

Catalog No. ABIN2044639

Human SNAP25 ELISA Kit Colorimetric assay for quantification of Human SNAP25.

Quick Overview for SNAP25 ELISA Kit (ABIN2044639)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details SNAP25 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.051 ng/mL

Application Details

Plate: Pre-coated

Protocol: Synaptosomal Associated Protein 25kDa (SNAP25) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synaptosomal Associated Protein 25kDa (SNAP25). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synaptosomal Associated Protein 25kDa (SNAP25). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synaptosomal Associated Protein 25kDa (SNAP25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Synaptosomal Associated Protein 25kDa (SNAP25) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: Synaptosomal Associated Protein 25kDa (SNAP25)

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport