SNCA ELISA Kit

Catalog No. ABIN2044643

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat SNCA.

Quick Overview for SNCA ELISA Kit (ABIN2044643)

Target: SNCA (Synuclein, alpha (SNCA))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details SNCA ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 5.4 pg/mL

Application Details

Plate: Pre-coated

Protocol: Synuclein Alpha (SNCa) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synuclein Alpha (SNCa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synuclein Alpha (SNCa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synuclein Alpha (SNCa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Synuclein Alpha (SNCa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: Synuclein alpha (SNCa)

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity