TIMD4 ELISA Kit

Catalog No. ABIN2044647

This Human TIMD4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human TIMD4.

Quick Overview for TIMD4 ELISA Kit (ABIN2044647)

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details TIMD4 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.062 ng/mL

Application Details

Plate: Pre-coated

Protocol: T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TIMD4

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Alternative Name: T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4)

Pathways: Cancer Immune Checkpoints