TAUT ELISA Kit
Quick Overview for TAUT ELISA Kit (ABIN2044653)
Target
See all TAUT ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.052 ng/mL
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Plate
- Pre-coated
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Protocol
- Taurine Transporter (TAUT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Taurine Transporter (TAUT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Taurine Transporter (TAUT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Taurine Transporter (TAUT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Taurine Transporter (TAUT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- TAUT (Taurine Transporter (TAUT))
Target See all TAUT ELISA Kits
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