TERT ELISA Kit

Catalog No. ABIN2044655

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat TERT.

Quick Overview for TERT ELISA Kit (ABIN2044655)

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12-200 ng/mL

Application: ELISA

Product Details TERT ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Sensitivity: < 1.08 ng/mL

Application Details

Plate: Pre-coated

Protocol: Telomerase Reverse Transcriptase (TERT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomerase Reverse Transcriptase (TERT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Telomerase Reverse Transcriptase (TERT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Telomerase Reverse Transcriptase (TERT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Telomerase Reverse Transcriptase (TERT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TERT

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Pathways: Telomere Maintenance