TFE3 ELISA Kit

Catalog No. ABIN2044683

This Human TFE3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human TFE3.

Quick Overview for TFE3 ELISA Kit (ABIN2044683)

Target: TFE3 (Transcription Factor E3 (TFE3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details TFE3 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.118 ng/mL

Application Details

Plate: Pre-coated

Protocol: Transcription Factor Binding To IGHM Enhancer 3 (TFE3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor Binding To IGHM Enhancer 3 (TFE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Transcription Factor Binding To IGHM Enhancer 3 (TFE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TFE3

Target: TFE3 (Transcription Factor E3 (TFE3))

Alternative Name: Transcription Factor Binding To IGHM Enhancer 3 (TFE3)