Transferrin Receptor 2 ELISA Kit

Catalog No. ABIN2044687

Human Transferrin Receptor 2 ELISA Kit Colorimetric assay for quantification of Human Transferrin Receptor 2.

Quick Overview for Transferrin Receptor 2 ELISA Kit (ABIN2044687)

Target: Transferrin Receptor 2 (TFR2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details Transferrin Receptor 2 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Sensitivity: < 29.4 pg/mL

Application Details

Plate: Pre-coated

Protocol: Transferrin Receptor 2 (TFR2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transferrin Receptor 2 (TFR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transferrin Receptor 2 (TFR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transferrin Receptor 2 (TFR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Transferrin Receptor 2 (TFR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Transferrin Receptor 2

Target: Transferrin Receptor 2 (TFR2)

Pathways: Transition Metal Ion Homeostasis