TGFB2 ELISA Kit

Catalog No. ABIN2044693

Rat TGFB2 ELISA Kit, Colorimetric assay for quantification of Rat TGFB2.

Quick Overview for TGFB2 ELISA Kit (ABIN2044693)

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details TGFB2 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 6.5 pg/mL

Application Details

Plate: Pre-coated

Protocol: Transforming Growth Factor Beta 2 (TGFb2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 2 (TGFb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 2 (TGFb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 2 (TGFb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Transforming Growth Factor Beta 2 (TGFb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TGFB2

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Alternative Name: Transforming Growth Factor beta 2 (TGFb2)

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus