TNNT3A ELISA Kit

Catalog No. ABIN2044715

This Colorimetric ELISA kit is designed for the quantitative measurement of Human TNNT3A.

Quick Overview for TNNT3A ELISA Kit (ABIN2044715)

Target: TNNT3A (Fast Skeletal Troponin T3a (TNNT3A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details TNNT3A ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 6.2 pg/mL

Application Details

Plate: Pre-coated

Protocol: Troponin T Type 3, Fast Skeletal (TNNT3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T Type 3, Fast Skeletal (TNNT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin T Type 3, Fast Skeletal (TNNT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin T Type 3, Fast Skeletal (TNNT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Troponin T Type 3, Fast Skeletal (TNNT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TNNT3A

Target: TNNT3A (Fast Skeletal Troponin T3a (TNNT3A))

Alternative Name: Troponin T Type 3, Fast Skeletal (TNNT3)