TAG-72 / CA72.4 ELISA Kit

Catalog No. ABIN2044719

The Human TAG-72 / CA72.4 ELISA Kit (ABIN2044719) is a Colorimetric ELISA Kit designed to quantify Human TAG-72 / CA72.4.

Quick Overview for TAG-72 / CA72.4 ELISA Kit (ABIN2044719)

Target: TAG-72 / CA72.4

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.102 ng/mL

Application Details

Plate: Pre-coated

Protocol: Tumor Associated Glycoprotein 72 (TAG72) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Associated Glycoprotein 72 (TAG72). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Associated Glycoprotein 72 (TAG72). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Associated Glycoprotein 72 (TAG72), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Tumor Associated Glycoprotein 72 (TAG72) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details

Target: TAG-72 / CA72.4