TNFAIP6 ELISA Kit

Catalog No. ABIN2044725

This Mouse TNFAIP6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse TNFAIP6.

Quick Overview for TNFAIP6 ELISA Kit (ABIN2044725)

Target: TNFAIP6 (Tumor Necrosis Factor-Inducible Protein 6 (TNFAIP6))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details TNFAIP6 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TNFAIP6

Target: TNFAIP6 (Tumor Necrosis Factor-Inducible Protein 6 (TNFAIP6))

Alternative Name: Tumor Necrosis Factor alpha Induced Protein 6 (TNFaIP6)