UQCR11 ELISA Kit

Catalog No. ABIN2044735

Human UQCR11 ELISA Kit Colorimetric assay for quantification of Human UQCR11.

Quick Overview for UQCR11 ELISA Kit (ABIN2044735)

Target: UQCR11 (Ubiquinol-Cytochrome C Reductase, Complex III Subunit XI (UQCR11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details UQCR11 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.29 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ubiquinol Cytochrome C Reductase (UQCR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquinol Cytochrome C Reductase (UQCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquinol Cytochrome C Reductase (UQCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquinol Cytochrome C Reductase (UQCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquinol Cytochrome C Reductase (UQCR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for UQCR11

Target: UQCR11 (Ubiquinol-Cytochrome C Reductase, Complex III Subunit XI (UQCR11))

Alternative Name: Ubiquinol Cytochrome C Reductase (UQCR)

Pathways: Proton Transport