UBR4 ELISA Kit

Catalog No. ABIN2044743

This Human UBR4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human UBR4.

Quick Overview for UBR4 ELISA Kit (ABIN2044743)

Target: UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details UBR4 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for UBR4

Target: UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))