UGT2B7 ELISA Kit

Catalog No. ABIN2044749

This Human UGT2B7 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human UGT2B7.

Quick Overview for UGT2B7 ELISA Kit (ABIN2044749)

Target: UGT2B7 (UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details UGT2B7 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.113 ng/mL

Application Details

Plate: Pre-coated

Protocol: UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for UGT2B7

Target: UGT2B7 (UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7))

Pathways: Steroid Hormone Biosynthesis