UPRT ELISA Kit

Catalog No. ABIN2044757

This Colorimetric ELISA kit is designed for the quantitative measurement of Human UPRT.

Quick Overview for UPRT ELISA Kit (ABIN2044757)

Target: UPRT (Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details UPRT ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.051 ng/mL

Application Details

Plate: Pre-coated

Protocol: Uracil Phosphoribosyltransferase (UPRT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uracil Phosphoribosyltransferase (UPRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uracil Phosphoribosyltransferase (UPRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uracil Phosphoribosyltransferase (UPRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Uracil Phosphoribosyltransferase (UPRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for UPRT

Target: UPRT (Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT))

Alternative Name: Uracil Phosphoribosyltransferase (UPRT)

Pathways: Ribonucleoside Biosynthetic Process