VASP ELISA Kit

Catalog No. ABIN2044785

This Rat VASP ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat VASP.

Quick Overview for VASP ELISA Kit (ABIN2044785)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details VASP ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.052 ng/mL

Application Details

Plate: Pre-coated

Protocol: Vasodilator Stimulated Phosphoprotein (VASP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vasodilator Stimulated Phosphoprotein (VASP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vasodilator Stimulated Phosphoprotein (VASP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: Vasodilator Stimulated Phosphoprotein (VASP)

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation